Tag: chromatin-accessibility

Type: All Skills Tools
skill ★ 34

ENCODE DNase-seq Pipeline for Hotspots and Footprints

This skill executes the comprehensive ENCODE DNase-seq pipeline, processing paired FASTQ reads to identify DNase hypersensitive sites (DHSs) and perform TF footprinting analysis. It utilizes Nextflow to generate critical data products, incl…

ammawla/encode-toolkit dnase-seq hotspots footprinting chromatin-accessibility
skill ★ 34

ENCODE ATAC-seq Pipeline for Chromatin Accessibility Analysis

This skill executes the comprehensive ENCODE ATAC-seq workflow, processing raw FASTQ files through alignment, Tn5 offset correction, and filtering. It generates high-quality peak calls and signal tracks suitable for detailed chromatin acces…

ammawla/encode-toolkit atac-seq chromatin-accessibility nextflow peak-calling
skill ★ 34

Aggregate comprehensive open chromatin accessibility maps

This skill builds a comprehensive union map of open chromatin regions by aggregating and merging ATAC-seq and DNase-seq narrowPeak data across multiple ENCODE experiments. It handles cross-platform variation, applies necessary filtering (su…

ammawla/encode-toolkit chromatin-accessibility atac-dnase peak-aggregation genomics
skill ★ 34

DNase-seq pipeline for accessibility and footprints

Executes a comprehensive DNase-seq workflow, processing paired-end FASTQ reads to identify DNase hypersensitive sites (DHSs) and perform transcription factor footprinting. The pipeline leverages Nextflow to manage alignment, peak calling vi…

ammawla/encode-toolkit dnase-seq chromatin-accessibility hotspot-calling footprinting
tool ★ 34

ENCODE ATAC-seq Pipeline for Chromatin Accessibility

This tool executes the full ENCODE ATAC-seq workflow, processing raw FASTQ files through alignment, Tn5 offset correction, and rigorous filtering. It generates nucleosome-free peaks and signal tracks following established ENCODE standards.

ammawla/encode-toolkit atac-seq chromatin-accessibility peak-calling nextflow
skill ★ 34

Integrate multi-omics for regulatory landscape

This skill integrates diverse ENCODE datasets, including RNA-seq, ATAC-seq, and various ChIP-seq assays, to construct a comprehensive regulatory landscape. It allows users to map cell-type-specific regulatory elements by correlating gene ex…

ammawla/encode-toolkit multi-omics epigenomics regulatory-network chromatin-accessibility
skill ★ 34

Comparing ENCODE Experiments Across Biosamples

This skill enables the systematic comparison of ENCODE experiments across various tissues, cell lines, and biosamples to identify tissue-specific regulatory patterns. It facilitates the identification of constitutive versus variable regulat…

ammawla/encode-toolkit encode epigenomics biosample-comparison tissue-specificity
skill ★ 34

Aggregate Chromatin Accessibility Peaks Across Studies

This skill generates a comprehensive union map of open chromatin by merging ATAC-seq and DNase-seq narrowPeak data from multiple ENCODE experiments. It handles cross-platform variation and applies rigorous filtering and confidence annotatio…

ammawla/encode-toolkit chromatin-accessibility atac-seq dnase-seq peak-aggregation